Abstract P30: The Mechanical Environment Modulates Lymphatic Tube Formation In Vitro

PURPOSE: The current gold standard for the repair of segmental bone defects is autologous bone grafts or flaps, however these are limited by donor site morbidity, limited graft tissue availability, and potential for complications at the harvest site. ASCs (adipose derived stem cells) have potential to aid bone regeneration due to their ability to differentiate into osteoblasts. BMP-2 (bone morphogenetic protein) has been shown to induce osteogenic differentiation of ASCs and enhance bone growth. We present a bioreactor for bone regeneration consisting of three main components: (i) a biodegradable PLLA scaffold seeded with ASCs, (ii) a drug reservoir made of PLLA and (iii) a controlled drug diffusion system for sustained release of BMP-2 to repair segmental bone defects. The drug reservoir is attached to the porous polymer scaffold seeded with ASCs and is capable of delivering growth factors for prolonged periods.